A late penalty by USA fly half Kimber Rozier proved the difference as Wales fell to a 7-10 defeat in their 2014 World Cup warm-up match at Pandy Park in Cross Keys.
Both sides scored one converted try apiece with winger Vanesha McGee ploughing over for USA and No 8 Sioned Harries replying in the second half for Wales.
Both sides ignored the wind and rain to play an open game of rugby with McGee and full back Akilaini Baravilala a constant threat with ball in hand for USA while Dyddgu Hywel and Laurie Harries featured regularly in counter attacks for Wales.
The teams were deadlocked until the 39th minute when USA ran the ball right and McGee dived over to score a well-executed try despite the best efforts of Harries and Hywel in defence. Rozier landed an excellent conversion to give the Americans a hard fought 7-0 lead at the interval.
Wales got back in to the game in the early stages of the second half through their forwards with Caryl Thomas and Catrin Edwards willing workhorses.
Wales got back on level terms in the 60th minute after holding onto possession for several phases. The ball was spread wide where Sioned Harries was lurking with intent and the speedy No 8 showed a clean pair of heels as she cut back against the grain to score under the posts. Laurie Harries added the extras to bring the scores level at 7-7.
Wales continued to attack but were guilty of coughing up possession at crucial times. USA made them pay by bursting downfield to set up Rozier for a penalty after Welsh forwards were penalised for coming in at the side of a ruck. The fly half had no trouble striking the ball between the uprights to edge her side back in front.
Wales finished the match in the ascendency but couldn't find a way through as USA held out for a gritty win.
Despite the narrow defeat, Wales Women's head coach Rhys Edwards was satisfied with his team's efforts.
"Going into the game we didn't know much about them but we knew it was going to be a tough game, especially as they are ranked in the top five teams of the world. The scoreboard doesn't really reflect that so I'm pretty pleased with the performance.
"We created some chances against one of the best teams in the world - the difference being when they get a chance they score, we don't. We take two, three, four or five times to get on the score board. We've got to show some composure and a bit of belief but we are certainly knocking on the door.
"We are improving and we showed that today. As we look ahead to the World Cup, we have another game against Ireland and there is no reason why we can't kick on from there and challenge in France."
WRU chief executive Martyn Phillips, WRU chairman Gareth Davies and Dragons chief executive Stuart Davies discuss the historic intentions of the governing body to assume operational control of the Newport Gwent Dragons, with a targeted completion date of 1st July.
Grand Slams, Triple Crowns and British and Irish Lions tours - Sir Gareth Edwards did it all. But what are his favourite moments in what was an amazing career? WRU TV finds out - along with his favourite players of today in the third and final instalment of our series with the greatest rugby player ever.
JPR Williams, Gerald Davies and Mervyn Davies were among those in the Welsh ranks when Sir Gareth Edwards began his international career against France nearly 50 years ago, but who was the player who took the young scrum half under their wing?
Wales head coach Rob Howley and captain Alun Wyn Jones are expecting a physical battle against France on Saturday but both are hoping for another impact from the bench as the men in red chase a top-four world ranking.
Ireland head coach Joe Schmidt believes the yellow card received by Johnathan Sexton was the crucial period of the game which handed victory to a 'clever' Wales side in a titanic RBS 6 Nations clash at Principality Stadium.
Man of the match Rhys Webb is delighted he and his team mates were able to produce a performance to make Welsh fans proud after Wales scored three tries en route to victory against a very good Ireland side at Principality Stadium.
All eyes on the Wales games against Ireland this weekend and we also hear from teams involved in the WRU Cup draw in the Principality Welsh Rugby Union Podcast. Coach Rob Howley, captain Alun Wyn Jones, back rowers Ross Moriarty and Sam Warburton on Wales, backs coach Nick Wakley and scrum half Keira Bevan on Wales Women, forwards coach Chris Horsman and prop Rhys Carre on Wales U20s, while we also hear from Merthyr, Pontypridd and Cross Keys about still being up for the Cup.
Wales head coach Rob Howley has kept faith with the side who suffered a disappointing defeat to Scotland by naming an unchanged side to face Ireland on Friday evening at Principality Stadium in the penultimate round of the RBS 6 Nations.
Ospreys and Wales duo Rhys Webb and Scott Baldwin return to their home town club Bridgend Ravens as they prepare for life after their rugby playing careers by undertaking their coaching badges at the Principality Premiership outfit.
Defence coach Richard Hodges and centre Keiran Williams discuss their U20 Six Nations campaign after victory against Scotland. Wales U20 are back at home in Parc Eirias this Saturday (18:30) to face Ireland in the penultimate round of the competition.
Sam Warburton realises a Six Nations title is now out of the question after the defeat to Scotland, but the former skipper says Wales will be playing for pride when Ireland visit Principality Stadium in the next round of the RBS 6 Nations.
Siwan Lillicrap, who featured for Wales throughout this year's Women's Six Nations, will have more investment in the Welsh Varsity than most of the many thousands of people who pour into Principality Stadium tomorrow.
Wales head coach Rowland Phillips believes Saturday's 39-19 defeat to France in Brive will prove an invaluable experience for his players as his side now prepare for the Women's Rugby World Cup which will be staged in Ireland this summer.