The Brave Blossoms have never beaten a major nation but they enter the second and final Test on the back of a sterling performance last weekend which saw them give Robin McBryde's side a big scare before Wales edged a hard fought encounter 18-22.
The Six Nations champions have made four changes for the finale while Japan welcome back captain Toshiaki Hirose, the right wing who has been out since April with injury.
"I'm really glad to be back. I'm excited to play a game. We lost the previous game by only four points. I really want to win this time," said Hirose.
Japan - Ayumu Goromaru; Toshiaki Hirose (capt), Male Sau, Craig Wing, Kenki Fukuoka; Harumichi Tatekawa, Fumiaki Tanaka; Takashi Kikutani, Michael Broadhurst, Hendrik Tui; Shoji Ito, Hitoshi Ono; Hiroshi Yamashita, Shota Horie, Masataka Mikami
Replacements: Takeshi Kizu, Yusuke Nagae, Kensuke Hatakeyama, Shinya Makabe, Justin Ives, Atsushi Hiwasa, Yu Tamura, Yoshikazu Fujita
Wales - Liam Williams; Harry Robinson, Owen Williams, Jonathan Spratt, Tom Prydie; Dan Biggar, Lloyd Williams; Rhys Gill, Emyr Phillips, Scott Andrews (Blues); Bradley Davies (capt), Lou Reed; James King, Josh Navidi, Andries Pretorius
Replacements: Scott Baldwin, Rhodri Jones, Craig Mitchell, Andrew Coombs, Dan Baker, Tavis Knoyle, Rhys Patchell, Dafydd Howells
You can follow the match live, by clicking the above link just before the 6am kick-off.