The Welsh team will run out to the Catatonia classic, 'International Velvet', before they take on England at the Millennium Stadium on Saturday.
The song sung by Cerys Matthews was the top choice of Welsh fans in a competition run by the Welsh Rugby Union in conjunction with Wales on Sunday and Real Radio.
"We were looking for a musical anthem to play before the Welsh team take to the field and we felt it was only right to consult the fans and hear from them what are their favourites," said WRU commercial manager Gwyn Thomas.
"We had a great response through our media partners and via our own website and the Catatonia classic was a clear winner over Eminem, Queen, the Sterophonics and Manic Street Preachers.
"The idea is for the fans to get behind the side as they emerge from the dressing rooms and take the field. I'm sure the fans will enjoy the moment and I'm sure they might join in with the chorus."
Catatonia first performed their 'International Velvet' song, which contains the phrase 'every day I wake up I thank the Lord I'm Welsh', before the opening game of the 1999 Rugby World Cup in Cardiff.
Any fans who still want to join in the fun at the Millennium Stadium, and see Stephen Jones' side take on the top team in the world, can still buy tickets on the day of the game.
Tickets will be available from booths at Gates 2, 3, 4 and 6/7 from Saturday morning. There are only 14,000 tickets left - Wales have sold 40,000 and England 20,000 - and the only categories remaining are in the Â£25 and Â£30 sections.
Please note that the Millennium Stadium Ticket Office will only be open for collections on the day of the game, and not for the sale of tickets.
UEFA has today confirmed the Football Association of Wales (FAW) has been successful in its bid for the Millennium Stadium to host the 2017 UEFA Champions League Final on Saturday 3rd June, 2017 (kick-off: 19.45hrs GMT).
The winners of a filmmaking competition organised by the education charity Into Film and the WRU enjoyed a behind the scenes tour of the Millennium Stadium - and a chance to be photographed with the WRU's fire-breathing, rugby loving mascot, Scorch The Dragon, and Welsh international, Jake Ball.
The Wales Under 20 camp are in a quietly confident mood as they face Ireland in the U20 Championship, with Coaching Co-Ordinator Allan Lewis and full back Dafydd Howells targeting a top five finish in the tournament.
Wales U20 may start as favourites against Japan this evening in the U20 Championship, but Team Manager Mark Taylor is warning against complacency. He is also hoping a good win will boost morale and 'kickstart' performances.
Coach Richard Hodges is hoping Wales U20 can put a disappointing campaign behind them when they tackle Japan tomorrow as they look to finish the U20 Championship strongly to secure a good seeding for next year's tournament.