Nigel Davies admitted Simon Easterby's yellow card cost his troops a valuable victory following the slender defeat against Glasgow Warriors.
The Scarlets trailed 13-11 when experienced flanker Easterby was harshly sin-binned for an altercation with Glasgow skipper Alastair Kellock in the 67th minute.
But Davies reckons he will have to read the riot act to his players in a bid to stop the ill-discipline reoccurring. He said: "I'm really annoyed because we deserved to pick up something from the game.
"We bombed a couple of guilt-edged opportunities in the second half but our discipline has become a huge issue again.
"We've made a big point of it but in our last two competitive games we have been penalised as a result.
"Simon's sin-bin was at a key stage of the game and the kick allowed Glasgow to put us out of reach of the bonus point.
"Simon has told me he was yellow-carded for retaliating and you can't afford to do that. It's a difficulty for him because he is a player and a coach and as coaches, we're adamant that our discipline will get better.
"There's a responsibility on all the players whether they are young or old. But we will have to sit down and talk about that because we can't play without 15 men on the field."
The Scarlets rallied in emphatic style after Rob Dewey's first half try at Firhill put the hosts in command. Full-back Dan Evans crossed before the break and Rhys Priestland kept the visitors in touch before Easterby was ordered off by Irish official John Lacey.
But the Scarlets chief insists there were positives to take following his side's first defeat of the season. "I'm hugely frustrated because it was a game we could and should have won. We didn't control the game in the first half but we showed plenty of endeavour and character and the line-out was a strong point for us. But we're just not helping ourselves."
Rhys Thomas, who was axed following his yellow-card last weekend, is expected to return for the Scarlets' home clash with Munster next Saturday.
As the countdown continues for RWC2015, Tournament Chief Executive Debbie Jevans visited the Millennium Stadium to discuss preparations for the eight matches and reveal the positive news that 100,000 tickets for games at the home of Welsh rugby have sold in the last seven days.
Jason Harries is targeting a first win on the World Series circuit this season with Wales. The series returns to action this weekend in Dubai where Harries is part of a team hoping to build from its efforts in round one in October.
Wales skipper Jevon Groves says his side have addressed a few points from Gold Coast and aim to 'hit the ground running' in the next two rounds of the World Series, kicking off with this weekend in Dubai before heading off to South Africa next week.
Wales Sevens manager Steff Thomas is hopeful his side can build from a quarter-final spot in the opening round of the World Series in Australia when his side take part in the forthcoming double header in Dubai and South Africa.