Cardiff Blues coach Dai Young is bracing himself for his side's biggest game of the season without his club captain.
Young watched on as skipper Paul Tito limped from the field in last weekend's 21-20 defeat to Magners League title holders Leinster.
Tito picked up a knee strain, and his inclusion in the squad that travel to Kingsholm for Sunday's crunch Heineken Cup clash is unlikely, according to Young who told the Western Mail: "Paul is very, very doubtful.
"It's very unlikely he will be fit for the Gloucester game, but he should be fit for the visit of Calvisano."
But Young has been told the injury will not keep Tito out of action for long. He explained: "It isn't a long-term problem, but he has strained his medial ligament in his knee."
The Blues still have work to do to qualify from Pool Six of the Heineken Cup and repeat last season's achievement of reaching the final eight.
Home advantage is crucial in European competition - as the Blues learnt when they were defeated by Toulouse last year. And the winners of the Kingsholm clash look likely to secure top spot and a home draw for the quarter-finals.
Cardiff have won all four of their matches to date, and have 18 points, meaning they sit top of the Pool - but have not yet confirmed their quarter-final qualification. They host Calvisano in the final match and would expect to take maxiumum points against a side who have failed to win a Heineken Cup match this season.
Gloucester's only defeat was at the hands of the Blues in Round 2, Cardiff "won ugly" on that day, according to Young. They would take another ugly result on Sunday.
Sam Cross and Luke Treharne have been named as co-captains of the Wales Sevens side as head coach Gareth Williams names his 12-man squad to play in the first leg of the HSBC World Series in Dubai next weekend.
Bedwas head coach Steve Law and players Nicky Griffiths and Alun Rees extol the virtues of the close knit club as they prepare to host third place RGC 1404 - who are one place head of them on the Principality Premiership points table - on Saturday.
Many of the Wales squad have said Diolch (thank you) to someone who played an important part in their early playing days by nominating them to be invited to the Wales v South Africa match. One person, Gowerton Comprehensive geography teacher Mr Dean Mason was nominated by two of his former pupils, Dan Biggar and Sam Davies.
The second WRU disability rugby pilot has been delivered, this time in Caerphilly schools, culminating in a festival at the Caerphilly Centre of Sporting Excellence, Ystrad Mynach tomorrow. Plans are in place to set up a Disability Rugby Club in Newbridge to provide long-term playing opportunities.
WRU TV: Hear from inside the Wales camp as Neil Jenkins discusses this weekend's clash with South Africa and how the squad are looking to put in a strong performance to round of the 2016 Under Armour Series