The International Rugby Board has confirmed that the North v South clash of the rugby hemispheres will take place at Twickenham on 30 November.
The Millennium Stadium bid for the game when the fixture was initially put out to tender, but the IRB have now opted to go to London for the inaugural fixture.
"The match was put out to tender, and the WRU duly submitted a bid. It was never at any stage formally agreed that it would be in Cardiff, and we're obviously disappointed to have missed out on this occasion," said WRU media manager Lyn Davies.
South Africa's Andre Watson has already been named as the referee for the game and the IRB will shortly announce a match sponsor and TV broadcaster.
Set to become a biennial event in the rugby calendar, the proceeds will go towards the development of rugby in the smaller playing countries around the globe.
IRB chief executive Mike Miller claims there is "tremendous enthusiasm" for the game.
The IRB will also hold their Annual Awards ceremony in London on the same day as the game.
Despite drawing with Ireland in the opening round of the RBS 6 Nations, Wales defence coach Shaun Edwards believes the championship 'is still in our hands' as he prepares his side to face Scotland at Principality Stadium tomorrow.
Meet the members of the WRU's inaugural Youth Board - after an online selection process which saw a host of high calibre candidates submit video applications the WRU has made its first 15 appointments.The board is made up of individuals aged between 16 and 21, and will meet for the first time at Principality Stadium on Monday 15th February.
The Wales Women's team headed to Dare Valley Country Park on a team bonding exercise as the entire squad got together for the very first time in preparation for this season's Six Nations championship which kicks off on Saturday with a clash against defending champions Ireland, in Donnybrook.