The International Rugby Board has confirmed that the North v South clash of the rugby hemispheres will take place at Twickenham on 30 November.
The Millennium Stadium bid for the game when the fixture was initially put out to tender, but the IRB have now opted to go to London for the inaugural fixture.
"The match was put out to tender, and the WRU duly submitted a bid. It was never at any stage formally agreed that it would be in Cardiff, and we're obviously disappointed to have missed out on this occasion," said WRU media manager Lyn Davies.
South Africa's Andre Watson has already been named as the referee for the game and the IRB will shortly announce a match sponsor and TV broadcaster.
Set to become a biennial event in the rugby calendar, the proceeds will go towards the development of rugby in the smaller playing countries around the globe.
IRB chief executive Mike Miller claims there is "tremendous enthusiasm" for the game.
The IRB will also hold their Annual Awards ceremony in London on the same day as the game.
The WRU has launched a campaign to create a long term legacy for Welsh club rugby by highlighting the advantages of volunteering. Rhian Edwards, a volunteer at Seven Sisters, has enjoyed many benefits of her volunteering at a grassroots rugby club including being part of the Rugby World Cup volunteer workforce after being nominated by the WRU - and the WRU is asking for more people to develop their 'Welsh rugby roots'.
Wales head coach Warren Gatland and captain Sam Warburton say their first prioirty on Sunday in the opening game of the World Cup is to win against Uruguay and not get overly concerned with points difference.
With only days to go until the biggest rugby competition in the world begins, rugby fans visiting Cardiff this autumn will be able to enjoy a special display at National Museum Cardiff as part of the celebrations surrounding the Rugby World Cup.
Geraint John has taken up the new role of WRU elite coach development manager having arrived in Wales last week. WRU TV caught up with him at the WRU rugby department's strategy day at the Millennium Stadium.