Scott Quinnell is troulbled by the same knee that he hurt last summer in Australia and will miss Saturday's match against Scotland at the Millennium Stadium.
"It is the same knee, but it is a new injury," said Quinnell. "I just need a few weeks rest before the Heineken Cup semi-final for Llanelli against Leicester."
However, there was good news for Wales as Llanelli fly half Stephen Jones came through his club's Principality Cup quarter-final and also Cardiff centre Jamie Robinson was back in action in another quarter-final.
Both Robinson and his brother Nicky, along with Mark Taylor (Swansea) and Ian Gough (Newport) were in training with Wales over the weekend and Nathan Budgett (Bridgend) is hoping to be fit after limping off against LLanelli at half-time last Saturday.
Quinnell was the only one of the 22 players of the squad beaten by England at Twickenham who did not train.
Ebbw Vale captain Damien Hudd picked up the biggest accolade of the night at the 11th Principality Premiership Awards Evening held at Principality Stadium, after leading his side to the title for the first time.
Wales assistant coach Robin McBryde is predicting a tough battle against England on Sunday despite the fact they may not be considered to be at full strength. Players will be out to impress Eddie Jones which makes England a dangerous proposition, he says.
Wales U20 captain Tom Phillips and performance psychologist Dr Rich Neil explain why spending three days at Monmouth RFC has further strengthened the togetherness of the squad ahead of the #WorldRugbyU20s
Alongside Scarlets flanker James Davies and Cardiff Blues centre Cory Allen, three Wales Sevens core players have made the extended training squad for GB Sevens. Wales Head coach Gareth Williams tells WRU TV's Graeme Gillespie why Luke Treharne, Sam Cross and Luke Morgan earned selection in the initial training squad for Rio.
Wales Sevens head coach Gareth Williams tells WRU TV's Graeme Gillespie he has had to overcome a number of injuries before naming a competitive squad for this weekend's penultimate round of the World Series in Paris.