Scott Quinnell is troulbled by the same knee that he hurt last summer in Australia and will miss Saturday's match against Scotland at the Millennium Stadium.
"It is the same knee, but it is a new injury," said Quinnell. "I just need a few weeks rest before the Heineken Cup semi-final for Llanelli against Leicester."
However, there was good news for Wales as Llanelli fly half Stephen Jones came through his club's Principality Cup quarter-final and also Cardiff centre Jamie Robinson was back in action in another quarter-final.
Both Robinson and his brother Nicky, along with Mark Taylor (Swansea) and Ian Gough (Newport) were in training with Wales over the weekend and Nathan Budgett (Bridgend) is hoping to be fit after limping off against LLanelli at half-time last Saturday.
Quinnell was the only one of the 22 players of the squad beaten by England at Twickenham who did not train.
GB7s women left their England base to train in Wales this week. Olympic Games hopeful Rachael Burford gives WRU TV's Graeme Gillespie her thoughts on Welsh teammate Jasmine Joyce - a 'one touch wonder'
The GB7s women's squad is currently training at Wales' Centre of Excellence where assistant coach Richie Pugh tells WRU TV's Graeme Gillespie some 'tough calls' will have to be made when it comes to finalising the 12 spots for Rio's Olympic Games.
Wales U20's Billy McBryde is looking forward to starting his first game against New Zealand this afternoon in the World Rugby U20 Championship where his side are looking to gain revenge from last week's pool defeat to the Kiwis.