In a curtain-raiser to the South Africa-New Zealand Tri-Nations match in Durban, Wales Under-19's lost 20-19 to a South African Schools Academy team.
"We outscored them by three tries to two and it was a game we should have won," said coach Dai Rees. "But we started poorly and gave away too much possession. Given a few more minutes we would have won."
The home side led 13-0 before fly half Matthew Jones dropped a goal and wing Richard Fussell scored a try.
However, it was 21-8 before wing Stephen Ireland and centre Gavin Evans crossed for tries and centre Lee Thomas added one conversion.
A late run by Fussell was halted in the corner flag and Wales just lost out.
The final two matches are against Northern Transvaal on Wednesday and then the SA Schools at Johannesburg on Saturday, the latter being a curtain-raiser to the South Africa-Australia Tri-Nations match.
As the countdown continues for RWC2015, Tournament Chief Executive Debbie Jevans visited the Millennium Stadium to discuss preparations for the eight matches and reveal the positive news that 100,000 tickets for games at the home of Welsh rugby have sold in the last seven days.
Jason Harries is targeting a first win on the World Series circuit this season with Wales. The series returns to action this weekend in Dubai where Harries is part of a team hoping to build from its efforts in round one in October.
Wales skipper Jevon Groves says his side have addressed a few points from Gold Coast and aim to 'hit the ground running' in the next two rounds of the World Series, kicking off with this weekend in Dubai before heading off to South Africa next week.
Wales Sevens manager Steff Thomas is hopeful his side can build from a quarter-final spot in the opening round of the World Series in Australia when his side take part in the forthcoming double header in Dubai and South Africa.