Trebanos, who were beaten finalists last season, have crossed for 27 tries in their eight league matches and will be confident of knocking out their opponents to keep the dream of a second successive final.
Cardiff University are one of only two students sides left in the competition and if they can beat Abercwmboi in the re-arranged round two tie, they will make the short trip to Caedelyn Park to meet Rhiwbina of Four South East. The Squirrels are currently flying high in their division and will prove tough opponents whoever they meet.
Cardiff Medicals have to see off Old Illtydians of Five South East before starting to think of an away trip to either Cefn Coed, also of Five South East, or Nelson who play in Four West.
Shotton Steel will host Bala of Two North if they can beat off the challenge from COBRA, from the same division.
Ties to be played weekend of 29 January, 2011
Aberaeron v Tenby / Fishguard
Amman United / Penlan v Burry Port
Blaenavon / Whiteheads v Usk / Chepstow
Cefn Coed / Nelson v Old Illtydians / Cardiff Medicals
Crumlin / Blackwood Stars v Senghenydd / Cwmbran
Hafodyrynys / Nantyglo v New Tredegar / Markham
Llanhilleth / Caerleon v Talywain / Monmouth
Llanidloes / Dolgellau v Newtown
Llanybydder v Nantgaredig / Pembroke Dock Quins
Maesteg Quins / Cefn Cribbwr v Bryncethin
Pontycymmer / Bridgend Sports v Cambrian Welfare / Porth
Rhiwbina v Abercwmboi / Cardiff University
Shotton Steel / COBRA v Bala
Trebanos v Ystradgynlais
Vardre / Alltwen v Neath Athletic / Pyle
Wattstown / Pontyclun v Tonyrefail / Maestec Celtic