Wales coach Jason Lewis (right) has been keeping a close eye on Dan Cottrell's Ospreys side along with the remaining three teams in the competition.
The Dragons and Scarlets will be out to protect their unbeaten starts in the second round of the WRU Senior Women's Regional competition on Wednesday.
In its first season, the competition was created as a stepping stone from club rugby to international Under 20s and senior rugby. Each of the four regions has a sprinkling of senior international players with the rest of the teams made up of club and age grade players.
In last week's opening clashes, the Dragons beat Cardiff Blues 16-13 with the last move of the game while in another close encounter, the Scarlets beat the Ospreys 14-10.
Blues second row Gemma Hallett, in a pack where hooker Rachel Cork and Vicki Gaylard impressed, said the last gasp defeat will make her side more determined this week to record their first victory against the Scarlets at Carmarthen Athletic.
"It was really disappointing to lose in the last minute, particularly as our defence had been so good for most of the game. It was a really good game - end to end stuff. We went into the game slight underdogs but pushed them all the way.
"We are determined to get back to winning ways but the Scarlets are a good team and it's going to be tough."
Scarlets key play maker Naomi Thomas is expecting the Blues to come at them with all guns blazing.
"It should be a good game, they only just lost to the Dragons so will be out to get their first win," she said.
The Dragons travel to Waunarlwydd where they will tackle a young Ospreys side who gave the Scarlets a good run for their money last week.
Flanker Lisa Newton and second row Shona Powell-Hughes will be looking to build on their impressive performances as Wales coach Jason Lewis has still to confirm his World cup squad and has made it clear there are still positions up for grabs.
WRU Women's Regional Fixtures Wednesday 28th April
Ospreys v Dragons at Waunarlwydd
Scarlets v Blues at Carmarthen Athletic
Wednesday 5th May
Blues v Ospreys at Pontyclun
Dragons v Scarlets at Cross Keys
Scarlets v Blues at Carmarthen Athletic, Wednesday 29th April, KO 7.30pm:
Wales Head Coach Rob Howley talks to WRU TV's Sebastian Barrett about the 36-man squad he has just named for the forthcoming Under Armour series, which includes two uncapped players, Ospreys duo Sam Davies and Rory Thornton.
Head coach Rowland Phillips is deligthed he can now call BT Sport Cardiff Arms Park home for his Wales Women's side, who launch their international season next month with games against Scotland and UK Armed Forces in the heart of the capital city.
Wales Sevens star Sam Cross has put his home town club of Brynmawr on the map after winning a silver medal at the Rio Olympics with Team GB. His club have now honoured him by naming their players bar after him, as WRU TV discovered at the official launch, recently.
One of the newest members of the Principality Premiership, Bargoed admit it's proving to be a steep learning curve at the highest level, but are determined to become a force to be reckoned with. WRU TV visited the club last weekend when they took on Llanelli.
Table-topping Aberavon travel to north Wales to tackle RGC 1404 as both teams defend unbeaten starts to the season in the Principality Premiership. WRU TV's Graeme Gillespie visited the Wizards to see how their preparations were going for the game of the weekend.
The Half a Game initiative is currently sweeping across Wales and proving a major success. The scheme aims to give every young player at least half a game every weekend. WRU TV recently visited a festival in Narberth where clubs have fully embraced the initiative.
Both Bargoed and Swansea were seeking their first win of the Principality Premiership season this afternoon. It proved a fruitful day for the hosts - not only did they win, but Bargoed claimed a try bonus point.