Wales coach Jason Lewis (right) has been keeping a close eye on Dan Cottrell's Ospreys side along with the remaining three teams in the competition.
The Dragons and Scarlets will be out to protect their unbeaten starts in the second round of the WRU Senior Women's Regional competition on Wednesday.
In its first season, the competition was created as a stepping stone from club rugby to international Under 20s and senior rugby. Each of the four regions has a sprinkling of senior international players with the rest of the teams made up of club and age grade players.
In last week's opening clashes, the Dragons beat Cardiff Blues 16-13 with the last move of the game while in another close encounter, the Scarlets beat the Ospreys 14-10.
Blues second row Gemma Hallett, in a pack where hooker Rachel Cork and Vicki Gaylard impressed, said the last gasp defeat will make her side more determined this week to record their first victory against the Scarlets at Carmarthen Athletic.
"It was really disappointing to lose in the last minute, particularly as our defence had been so good for most of the game. It was a really good game - end to end stuff. We went into the game slight underdogs but pushed them all the way.
"We are determined to get back to winning ways but the Scarlets are a good team and it's going to be tough."
Scarlets key play maker Naomi Thomas is expecting the Blues to come at them with all guns blazing.
"It should be a good game, they only just lost to the Dragons so will be out to get their first win," she said.
The Dragons travel to Waunarlwydd where they will tackle a young Ospreys side who gave the Scarlets a good run for their money last week.
Flanker Lisa Newton and second row Shona Powell-Hughes will be looking to build on their impressive performances as Wales coach Jason Lewis has still to confirm his World cup squad and has made it clear there are still positions up for grabs.
WRU Women's Regional Fixtures Wednesday 28th April
Ospreys v Dragons at Waunarlwydd
Scarlets v Blues at Carmarthen Athletic
Wednesday 5th May
Blues v Ospreys at Pontyclun
Dragons v Scarlets at Cross Keys
Scarlets v Blues at Carmarthen Athletic, Wednesday 29th April, KO 7.30pm:
Wales U20 captain Tom Phillips and performance psychologist Dr Rich Neil explain why spending three days at Monmouth RFC has further strengthened the togetherness of the squad ahead of the #WorldRugbyU20s
Alongside Scarlets flanker James Davies and Cardiff Blues centre Cory Allen, three Wales Sevens core players have made the extended training squad for GB Sevens. Wales Head coach Gareth Williams tells WRU TV's Graeme Gillespie why Luke Treharne, Sam Cross and Luke Morgan earned selection in the initial training squad for Rio.
Wales Sevens head coach Gareth Williams tells WRU TV's Graeme Gillespie he has had to overcome a number of injuries before naming a competitive squad for this weekend's penultimate round of the World Series in Paris.
Merthyr were officially crowned champions of the SSE SWALEC Championship at Dowlais on Saturday where captain Andy Powell paid tribute to his side who have earned promotion to the Principality Premiership
Coleg y Cymoedd's season has had a spectacular ending with victory in two cup finals and a massive victory over one of the best rugby playing schools in the world - Welsh coach Warren Gatland's old alma mater, Hamilton Boys' High School.
WRU chief executive Martyn Phillips hosted the second meeting of the WRU Youth Board last weekend ahead of the Judgement Day IV regional double-header. Tthe board, made up of 15 under-21-year-olds from around Wales, heard presentations from head of participation Ryan Jones, policy and integrity manager Jeremy Rogers and national women's rugby manager Caroline Spanton, with Pro Rugby Wales CEO Mark Davies also on hand to hear the range of views aired.
Wales duo Rhun Williams and Liam Belcher, who makes a welcome return after five months of injury woe, look ahead to next month's World Rugby U20 Championship in Manchester where they will face Ireland, Georgia and New Zealand.