The fixtures include a North Wales derby, with Ruthin drawing Dolgellau, premier division teams Neath and Cardiff Quins meet once again and first division Tylorstown and Blaunau Gwent face premier division Pontyclun and UWIC respectfully.
Following these second round fixtures, the Cup and Plate semi finals will be played on neutral territory on Sunday 14th March with the two finals on Sunday 28th March.
Cup Round 2 - Sunday 7th March 2010
Ruthin v Dolgellau
Neath Athletic v Cardiff Quins
Tylorstown v Pontyclun
Blaeanu Gwent v UWIC