Gethin Jenkins leaves the field against Scotland last Saturday.
Wales physio Mark Davies has today confirmed Matthew Rees, Gethin Jenkins and Gareth Williams will all be unavailable for selection for the RBS 6 Nations clash with France at the Millennium Stadium a week on Friday (Kick-off 8pm)
Scarlets hooker Rees has failed to recover from a groin problem, Blues prop Gethin Jenkins has a calf injury and fellow Blues player, hooker Gareth Williams, has damaged knee ligaments.
"Matthew (Rees) is progressing well and will continue his rehab in camp with Wales," said Davies.
"He will undergo exhaustive testing over the next few days which will give us a clearer idea of when he may be available, but we can confirm that the France game will come too soon for him.
"Gethin (Jenkins) has been scanned since leaving the field against Scotland and, as we thought, he has aggravated the calf injury which kept him out against England. At this stage we are not putting a time limit on his recovery, but again we know he will not take the field against France.
"Gareth (Williams) sustained medial ligament damage to his knee during the win over Scotland last weekend. He will undertake a rest and rehab programme and his progress will be re-assessed next week, but the injury rules him out of contention for Friday night."
As the countdown continues for RWC2015, Tournament Chief Executive Debbie Jevans visited the Millennium Stadium to discuss preparations for the eight matches and reveal the positive news that 100,000 tickets for games at the home of Welsh rugby have sold in the last seven days.
Jason Harries is targeting a first win on the World Series circuit this season with Wales. The series returns to action this weekend in Dubai where Harries is part of a team hoping to build from its efforts in round one in October.
Wales skipper Jevon Groves says his side have addressed a few points from Gold Coast and aim to 'hit the ground running' in the next two rounds of the World Series, kicking off with this weekend in Dubai before heading off to South Africa next week.
Wales Sevens manager Steff Thomas is hopeful his side can build from a quarter-final spot in the opening round of the World Series in Australia when his side take part in the forthcoming double header in Dubai and South Africa.