Head Coach Richard Hodges said, "Our disappointing league form means this quarter final is the biggest game of our season, so everything is to play for on Saturday."
The Wanderers take on a Quins side who won at The Memorial Ground only a few weeks ago, so shall be looking to avenge that league loss with a Cup semi final place.
Flying winger Rory Budina returns to the backline after scoring as a replacement against Pontypridd with in form scrum half Rhys Dyer retaining the number nine jersey.
In the pack Hodges gives starts to hooker Ieuan Davies returning from Wales U20's duty and Josh Navidi to help bolster the back row. Nick Kelly returns to the side in the second row to partner Tom Evans while Gareth Knight switches to the backrow.
Glamorgan Wanderers squad:
Backs: Owen Marshall, Rory Budina, Steve Cullen, Robin Lock, Ryan Healy, Scott Sneddon, Rhys Dyer, Gareth Morris, Tom Rowlands.
Forwards: Nathan Trevett, Ieuan Davies, Mark O'Leary, Tom Evans, Nick Kelly, Gareth Knight, Josh Navidi, Lee Highgate (Captain), Jimmy Richards, Ryan Beddows, Richard Thomas, Carl Kimber, Jon Barley