Jean De Villiers is out of action for eight weeks.
The Springboks will be without skipper Jean de Villiers for the two Test Castle Lager Series against Wales next month.
The Stormers centre underwent surgery on a knee injury last weekend and will be out of action for eight weeks. He hopes to return for the start of Rugby Championship in August.
"Losing our captain is a massive setback," said Springbok coach Heyneke Meyer. "Jean has been superb as our leader on and off the field and he was in excellent form as well.
"He is a wonderful ambassador for South Africa and is respected across the world as a leader - we will certainly miss him next month. His injury is the latest in a string of setbacks we've experienced, and with Jaque Fourie also ruled out it means we'll play with a new captain and a new midfield combination next month.
"Luckily we have good depth in the squad at centre, but replacing your captain is always difficult. We have a number of good leaders in the squad, but I don't want to rush a decision on the captaincy as we still have to finalise the squad for June."
De Villiers' place in the training squad that gathered in Durban on Sunday has been taken by promising Sharks lock Stephan Lewies. The Springboks will face a World XV at Newlands before they tackle Wales on successive weekends.
"It's great to see the excitement on the faces of the players, not only the uncapped guys, but also experienced Springboks," said Meyer.
"Everyone knows this is a very important season and with 18 matches left until we depart for the Rugby World Cup, the players are keen to impress and fight for positions in the squad. Our focus for now, however, is on the World XV match and ensuring we start the season on a positive note."
WRU TV were on hand at the launch of a major new commercial deal with WRU and Mercedes-Benz South Wales (part of the Sinclair Group), which sees the family company become an official supplier to the Welsh Rugby Union
On the back of the recent Women's Rugby World Cup, the WRU has launched a revitalised Women's and Girls domestic seasonal structure, and the future is looking bright.
Go to www.wru.co.uk/tryourgame for more information
Roger Lewis talks to WRU TV at the announcement of a new £60 million Rugby Services Agreement (RSA) which provides a new and positive contractual relationship between the WRU and its four Regions for the next six years has been agreed.
The Welsh Rugby Union has announced a major new sponsorship deal with Gullivers Sports Travel which will make the UK's leading, and longest established, multi-sport tour operator, an Official Supplier for the rest of the decade.
Having taken part in seven World Cups as a player and coach, Liza Burgess hopes to go out in style this afternoon when Wales take on Australia in the Play-off for seventh and eighth place in the Women's Rugby World Cup in Paris.
WRU Head of Rugby Josh Lewsey met with volunteers across the game earlier this week, championing their role in the sport. He was joined by Wales captain Sam Warburton for part of the day as volunteers took part in a 'Try Out' event for the 2015 Rugby World Cup
Scarlets skipper Ken Owens said his players have "taken it on the chin" from the coaches in the wake of last weekend's six-try defeat in Dublin and are ready to made amends against Benetton Treviso on Saturday night.
The massive strides Wales Under 18 Girls have made under the guidance of Rhys Edwards and Richie Pugh was fully illustrated when they finished runners-up to England in last weekend's Rugby Europe Under 18 tournament in Enkoping, Sweden.