Talismanic Italy skipper Sergio Parisse will miss Saturday's showdown against Wales after being suspended for 30 days.
Parisse will be free to play again on 18 March, after seeing red last weekend playing for his club Stade Francais against Bordeaux-Begles when he was dismissed for allegedly insulting match referee Laurent Cardona.
The ban, 40 days with 10 suspeneded, rules him out of Italy's remaining RBS 6 Nations games this season against Wales, England and Ireland.
The No 8 was sent off after 36 minutes of Stade's home match with Bordeaux-Begles, having scored a try in the fourth minute.
Italy coach Jacques Brunel had already called veteran flanker Mauro Bergamasco into his squad to face Wales.
The last five years have been an emotional rollercoaster on and off the pitch for RGC1404. Promotion to the Principality Premiership was the culmination of five years of hard work and investment in North Wales. WRU TV caught up with prop Joe Simpson, who was one of the first intake of WRU North Wales Academy players in 2010.
Ebbw Vale captain Damien Hudd picked up the biggest accolade of the night at the 11th Principality Premiership Awards Evening held at Principality Stadium, after leading his side to the title for the first time.
Wales assistant coach Robin McBryde is predicting a tough battle against England on Sunday despite the fact they may not be considered to be at full strength. Players will be out to impress Eddie Jones which makes England a dangerous proposition, he says.
Wales U20 captain Tom Phillips and performance psychologist Dr Rich Neil explain why spending three days at Monmouth RFC has further strengthened the togetherness of the squad ahead of the #WorldRugbyU20s