Cross Keys, Neath, Aberavon, Llanelli, Pontypridd and Swansea will fly the flag for Wales in a tournament which will kick off with a clash of previous winners when Bristol Rugby travel to Cornish Pirates.
"We have always aspired to play in the British & Irish Cup and kicking-off up in Scotland is fantastic," said Keys head coach Greg Woods.
"There has been a real buzz around the village and club about playing in the tournament and we are treating the matches as our marquee fixtures - we want to make a real impact in the competition."
The third British & Irish Cup will see a change in format, with a cross-Pool fixture list which guarantees two home fixtures for each side and with all English ties played in midweek to ease potential weekend fixture congestion.
Pool 1: Ayr, Cornish Pirates, Moseley, Neath,
Pool 2: Bristol Rugby, Cross Keys, Plymouth Albion, Munster
Pool 3: Aberavon, Bedford Blues, Leinster, London Scottish
Pool 4: Esher Rugby, Llanelli, London Welsh, Melrose
Pool 5: Currie, Doncaster Knights, Rotherham Titans, Pontypridd
Pool 6: Leeds Carnegie, Nottingham Rugby, Swansea, Ulster,
Round 1 (September 21): Cornish Pirates v Bristol Rugby, Moseley v Plymouth, London Scottish v London Welsh, Bedford Blues v Esher, Nottingham Rugby v Doncaster Knights, Leeds Carnegie v Rotherham Titans. (November 19): Neath v Munster, Ayr v Cross Keys, Aberavon v Llanelli, Leinster v Melrose, Currie v Ulster, Pontypridd v Swansea
Round 2 (October 12): Plymouth v Cornish Pirates, Bristol Rugby v Mosley, Esher v London Scottish, London Welsh v Bedford Blues, Doncaster Knights v Leeds Carnegie, Rotherham Titans v Nottingham Rugby. (December 10): Munster vs. Ayr, Llanelli v Leinster, Ulster v Pontypridd, Cross Keys v Neath, Melrose v Aberavon, Swansea v Currie
Round 3 (November 12): Cross Keys v Moseley, Munster v Cornish Pirates, Bristol Rugby v Neath, Plymouth v Ayr, Melrose v Bedford Blues, Llanelli v London Scottish, London Welsh v Aberavon, Esher v Leinster, Swansea v Rotherham Titans, Ulster v Doncaster Knights, Leeds Carnegie v Currie, Nottingham Rugby v Pontypridd
Round 4 (December 17): Mosley v Munster, Cornish Pirates v Cross Keys, Neath v Plymouth, Ayr v Bristol Rugby, Bedford Blues v Llanelli, London Scottish v Melrose, Aberavon v Esher, Leinster v London Welsh, Rotherham Titans v Ulster, Doncaster Knights v Swansea, Currie v Nottingham Rugby, Pontypridd v Leeds Carnegie
Quarter finals: January 21, 2012
Semi finals: April 7
Final: April 28