Centre Andrew Bishop is the only member of the backline to be named in successive starting XVs, while Alun Wyn Jones and Filo Tiatia maintain their places up front, although Tiatia switches from the blindside flank to No8.
Daniel Biggar starts at No 10, alongside new scrum-half signing Jamie Nutbrown, while David Bishop makes his first competitive appearance for the Ospreys since September 2005, when he suffered a fractured kneecap playing against Munster.
Prop Paul James captains the side in the absence of Ryan Jones as he equals Barry Williams' record of 101 appearances for the region.
Flanker Ben Lewis is recovering well after undergoing ACL reconstruction surgery to his right knee and is on target for a November return, while Gavin Henson should be back in action in early October following keyhole surgery on damaged ankle ligaments.
Fellow Welsh international Mike Phillips could also return early next month after undergoing surgery to repair damage to his anterior cruciate and medial collateral ligaments.
Richard Hibbard is recovering well after recently undergoing minor surgery on his shoulder and is hopeful of being available for selection before the end of September and Lyndon Bateman has been experiencing a recurring back injury and misses the first week of the season.
Ospreys (vs Glasgow, Friday September 12, kick-off 7.30pm)
15 Gareth Owen
14 Johnny Vaughton
13 David Bishop
12 Andrew Bishop
11 Nikki Walker
10 Daniel Biggar
9 Jamie Nutbrown
8 Filo Tiatia
7 Marty Holah
6 Tom Smith
5 Alun Wyn Jones
4 Ian Gough
3 Cai Griffiths
2 Ed Shervington
1 Paul James (capt)
Replacements
16 Huw Bennett
17 Duncan Jones
18 Andy Lloyd
19 Jonathan Thomas
20 Rhys Webb
21 James Hook
22 Kristian Phillips
