The Ospreys have been boosted by the surprise return of Nikki Walker and Jamie Nutbrown to their squad for the EDF Energy Cup tie with Worcester this Sunday.
Scotland wing Walker suffered a broken hand in the Heineken Cup loss at Leicester two weeks ago while Nutbrown also returns following a foot injury.
Gavin Henson is expected to start after his disciplinary problems and after only playing one match this season, will be desperate to get some game time ahead of the Invesco Perpetual Autum Series, having only played one full match this season.
Duncan Jones said: "We had a tough game against Harlequins and the win was all important. We do not want to let go off this trophy easily as champions.
"We recorded a good win away to Worcester last season but we will not be complacent. They are a totally different side, we understand it will be physical but we're up for the challenge and looking for a win."
Ospreys (from): Backs: Lee Byrne, Nikki Walker, Tommy Bowe, Shane Williams, Andrew Bishop, Gavin Henson, Ashley Beck, Dan Biggar, James Hook, Rhys Webb, Jamie Nutbrown. Forwards: Duncan Jones, Adam Jones, Andrew Millward, Richard Hibbard, Huw Bennett, Lyndon Bateman, Alun Wyn Jones, Ian Gough, Ryan Jones, Tom Smith, Filo Tiatia, Steve Tandy.
Welsh players and management joined Deputy Minister for Sport Ken Skates at the Pierhead Building, Cardiff Bay earlier this week to reveal the Union's pioneering school-club hub initiative has been so successful the project will now almost double the scale of the original project.
The Scarlets, Newport Gwent Dragons, Cardiff Blues and the Ospreys look ahead to Judgement Day III which sees the Blues take on the Ospreys (2.30) and the Dragons tackle the Scarlets (4.45pm) at the Millennium Stadium tomorrow afternoon.
Ystradgynlais and Ynysddu played out a titanic struggle at Taffs Well in the SSE SWALEC Bowl semi-final. At the end of the game neither team could be separated after a 15-15 stalemate but Ystradgynlais go through on try count.
Blues strike first in JD III battle - Newport Gwent Dragons lock Andrew Coombs, WRU referee Dylan Llyr Jones and Cardiff Blues hooker Matthew Rees decide dressing room allocations for Judgement Day III
Wales Under 18 captain Calum Haggett has received a conditional offer to read Biomedical Science at St Anne's College, Oxford. WRU chairman Gareth Davies went to meet Calum and Coleg y Cymoedd Principal Judith Evans to wish him luck in his exams and pass on some of his personal experience.
Dodgy tattoos, carthorses, Avatar and soft chins are revealed by Wales Sevens players Tom Williams and Sam Cross as they profile the Wales Sevens squad taking part in Hong Kong this weekend in the latest round of the HSBC Sevens World Series.