The Ospreys have been boosted by the surprise return of Nikki Walker and Jamie Nutbrown to their squad for the EDF Energy Cup tie with Worcester this Sunday.
Scotland wing Walker suffered a broken hand in the Heineken Cup loss at Leicester two weeks ago while Nutbrown also returns following a foot injury.
Gavin Henson is expected to start after his disciplinary problems and after only playing one match this season, will be desperate to get some game time ahead of the Invesco Perpetual Autum Series, having only played one full match this season.
Duncan Jones said: "We had a tough game against Harlequins and the win was all important. We do not want to let go off this trophy easily as champions.
"We recorded a good win away to Worcester last season but we will not be complacent. They are a totally different side, we understand it will be physical but we're up for the challenge and looking for a win."
Ospreys (from): Backs: Lee Byrne, Nikki Walker, Tommy Bowe, Shane Williams, Andrew Bishop, Gavin Henson, Ashley Beck, Dan Biggar, James Hook, Rhys Webb, Jamie Nutbrown. Forwards: Duncan Jones, Adam Jones, Andrew Millward, Richard Hibbard, Huw Bennett, Lyndon Bateman, Alun Wyn Jones, Ian Gough, Ryan Jones, Tom Smith, Filo Tiatia, Steve Tandy.
As the countdown continues for RWC2015, Tournament Chief Executive Debbie Jevans visited the Millennium Stadium to discuss preparations for the eight matches and reveal the positive news that 100,000 tickets for games at the home of Welsh rugby have sold in the last seven days.
Jason Harries is targeting a first win on the World Series circuit this season with Wales. The series returns to action this weekend in Dubai where Harries is part of a team hoping to build from its efforts in round one in October.
Wales skipper Jevon Groves says his side have addressed a few points from Gold Coast and aim to 'hit the ground running' in the next two rounds of the World Series, kicking off with this weekend in Dubai before heading off to South Africa next week.
Wales Sevens manager Steff Thomas is hopeful his side can build from a quarter-final spot in the opening round of the World Series in Australia when his side take part in the forthcoming double header in Dubai and South Africa.