The Ospreys have been boosted by the surprise return of Nikki Walker and Jamie Nutbrown to their squad for the EDF Energy Cup tie with Worcester this Sunday.
Scotland wing Walker suffered a broken hand in the Heineken Cup loss at Leicester two weeks ago while Nutbrown also returns following a foot injury.
Gavin Henson is expected to start after his disciplinary problems and after only playing one match this season, will be desperate to get some game time ahead of the Invesco Perpetual Autum Series, having only played one full match this season.
Duncan Jones said: "We had a tough game against Harlequins and the win was all important. We do not want to let go off this trophy easily as champions.
"We recorded a good win away to Worcester last season but we will not be complacent. They are a totally different side, we understand it will be physical but we're up for the challenge and looking for a win."
Ospreys (from): Backs: Lee Byrne, Nikki Walker, Tommy Bowe, Shane Williams, Andrew Bishop, Gavin Henson, Ashley Beck, Dan Biggar, James Hook, Rhys Webb, Jamie Nutbrown. Forwards: Duncan Jones, Adam Jones, Andrew Millward, Richard Hibbard, Huw Bennett, Lyndon Bateman, Alun Wyn Jones, Ian Gough, Ryan Jones, Tom Smith, Filo Tiatia, Steve Tandy.
GB7s women left their England base to train in Wales this week. Olympic Games hopeful Rachael Burford gives WRU TV's Graeme Gillespie her thoughts on Welsh teammate Jasmine Joyce - a 'one touch wonder'
The GB7s women's squad is currently training at Wales' Centre of Excellence where assistant coach Richie Pugh tells WRU TV's Graeme Gillespie some 'tough calls' will have to be made when it comes to finalising the 12 spots for Rio's Olympic Games.
Wales U20's Billy McBryde is looking forward to starting his first game against New Zealand this afternoon in the World Rugby U20 Championship where his side are looking to gain revenge from last week's pool defeat to the Kiwis.