Front-rower Mario Ledesma returns to international duty after flying in from Italy, along with halfback brothers Nicolas and Juan Fernandez Miranda.
The trio replace Pablo Cardinali, Lucio Lopez and German Bustos as the Pumas seek a Test series win in Buenos Aires.
Argentina held off a late Wales surge to record a 50-44 win in the first Test in Tucuman on Saturday.
Backs: Matias Albina, Lucas Borges, Manuel Contepomi, Felipe Contepomi, Martin Gaitan, Ignacio Fernandez Lobbe, Nicolas Fernandez Miranda, Juan Fernandez Miranda, Jose Orengo, Jose Maria Nunez Piossek, Hernan Senillosa.
Forwards: Patricio Albacete, Pablo Bouza, Martin Durand, Eusebio Guinazu, Omar Hasan, Mario Ledesma, Gonzalo Longo, Federico Mendez, Lucas Ostiglia, Rodrigo Roncero, Martin Schusterman.