Ospreys man Biggar again partners Lloyd Williams at half back tomorrow and after missing out on a late call up for the British & Irish Lions, he will be hoping for another solid performance.
The Six Nations-winning fly-half was overlooked by Warren Gatland this week who instead called up England's Billy Twelvetrees to provide back line cover for the injury-hit Lions in Australia.
Now McBryde has backed Biggar to put the blow behind him and help Wales seal a 2-0 series win.
"The initial Lions selection suggested they left enough room to invite one or two more as there are always injuries," said McBryde.
"But all Dan can do is prove a point on Saturday and hopefully we, as a team, will benefit from that.
"We've asked him to put his stamp on this game. We've given him a licence to step up, take on his role as a leader within the team and play things as he sees it."
McBryde has made four changes, including one positional, for tomorrow's game after what was at times, an unconvincing 22-18 win in Osaka last week.
Cardiff Blues flanker Josh Navidi will make his international debut at the Prince Chichibu Memorial Stadium, with Tom Prydie, Rhys Gill, and Andries Pretorius also featuring from the off.
The only change in the front row sees Saracens prop Gill come into the side alongside Emyr Phillips and Scott Andrews.
Captain Bradley Davies continues his partnership with regional teammate Lou Reed in the second row while in the back row, James King switches to blindside, Navidi comes in at openside flanker and Pretorius starts for the first time after impressing from the bench last week.
There is just one change in the backline where Prydie comes onto the wing in place of Dafydd Howells.
McBryde added: "It would be a setback to lose. We came here as a young group of players and coaches and everyone has stepped up, considering how many players and back-room staff have gone with the Lions.
"It is a great learning curve for us all but we need to finish on a high with a good quality performance otherwise there will be too much unsaid from our point of view.
"We need to get our own house in order, start the game better and be more accurate. I believe we'll do that because we've learned lessons and the players have addressed them this week.
"The intention of this tour was to identify team leaders, ask them to step up and take on extra responsibility and they have done that this week."
McBryde's selection of Navidi, a specialist openside, comes after Wales struggled at the breakdown during the first Test.
And the former Wales hooker is hopeful his introduction will help Wales to secure plenty of ball for their exciting backs.
"I don't regret not having Josh involved last week but you learn from any decision you make," he added.
"The contact area is a shared responsibility and as a back-row unit, we were not as combative as we wanted but this could be a more well-balanced back-row."
Japan coach Eddie Jones has made just the one change, fit-again captain Toshiaki Hirose returning to the side.
"We were in a position to win the last game but we weren't quite good enough," said Jones.
"But the great thing about playing two Tests is that you get another chance."