"There has been a lot of straight talking between the coaches and players and just how important the Welsh jersey is - I am sure you will see a performance everyone can be proud of," said Howley at the captain's run at the Millennium Stadium.
"It is a great accolade for Ryan to be captaining Wales for a record 29th and he will, as always, lead from the front."
Wales have made eight changes for the Samoan contest with Howley adding "the six-day turnaround was a factor.
"But it is also the opportunity for us to look at players who are in form for their region and to see if they can transfer that form to the international stage.
"The focus is on beating Samoa but it is about the big picture and the chance for the likes of Paul James, Dan Biggar, Ashley Beck and Justin Tipuric.
"Samoa are going to be a tough challenge but we were never going to pick the same 15 and we have always made changes for the second match of the autumn series."
"We have possibly made more changes than originally planned but we have to look at the four games and, for example, James (Hook) will not be available to us for the Australia game so we have to look at other options."
Jones, who will pack down at blindside flanker for his record breaking 29th match as skipper, added: "We have to put a few things right tactically and technically and get back to winning ways.
"We need to get a good result under our belt before facing New Zealand and Australia."
After the Samoa challenge Wales face the reigning world champions All Blacks and the Wallabies on successive Saturdays.
And with the IRB rankings for the 2015 World Cup finalised early next month Wales have plenty to play for and put the disappointment of the Pumas match firmly behind them.